Having held the position for more than a year, a friendly loss to Italy means the world and European champions have been replaced by the Oranje, who become just the seventh team to head the rankings.
Uruguay's Copa America success means they are now the top South American side in fifth, with Brazil dropping down to sixth.
Germany remain in third and England move up to fourth, while the make-up of the top 10 remains the same, with Italy, Portugal, Argentina and Croatia the other sides.
The Republic of Ireland mirror England in moving up two places, in their case to 31st, while Scotland are up six to 55th and Northern Ireland up three to 59th.
Wales' ranking does slide, though, with Gary Speed's men dropping five places to 117th, with Haiti moving above them in the standings.
Support the USA national team with an official 2013 Centennial soccer jersey from Nike. Its Official.
Jamaica is in need of a win against the U.S. Get the jersey of the Reggae Boyz.
The Nike Hypervenom is the latest cleat silo from Nike. It will change your game and is worn by the game's top players.
Germany is one of the top teams ever and they will play the US on Sunday.
Manchester City will try and win back the Premier League title with this new soccer jersey from Nike!
England will debut their new away jersey against Brazil at the Maracana Stadium on Sunday. Get in the Gear
Neymar's move from Santos to Barcelona is no doubt the biggest summer transfer.
The hoop soccer jersey is classic and cool. The USA hooped jersey is perfect for every fan. Get in the Gear.
The USA will wear their blue away jersey against Jamaica in their World Cup qualifier on Friday night.
The adidas Nitrocharge is the most talked about new cleat in years. It was released on May 15 and will take your pitch by storm.